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Regulatory
Part:BBa_K3431036:Design
Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang Group: iGEM20_CSMU_Taiwan (2020-10-17)
zz146_B Toehold Switch for miR-146 Detection
Subparts|Ruler|SS|DSScars: Show|HideVertical: Show|HideLength: UnknownGet part sequenceView plasmid
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Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure their minimal free energy are in an appropriate range (lower than 0 kcal/mole)
Source
This is a synthetic sequence based upon Pardee, K. et al. for detection of miRNA-31. We obtained this sequence through custom gene synthesis of IDT.